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- Title
An effective protein extraction method for two-dimensional electrophoresis in the anticancer herb A ndrographis paniculata Nees.
- Authors
Talei, Daryush; Valdiani, Alireza; Puad, Mohd Abdullah
- Abstract
Proteomic analysis of plants relies on high yields of pure protein. In plants, protein extraction and purification present a great challenge due to accumulation of a large amount of interfering substances, including polysaccharides, polyphenols, and secondary metabolites. Therefore, it is necessary to modify the extraction protocols. A study was conducted to compare four protein extraction and precipitation methods for proteomic analysis. The results showed significant differences in protein content among the four methods. The chloroform-trichloroacetic acid-acetone method using 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ( HEPES) buffer provided the best results in terms of protein content, pellets, spot resolution, and intensity of unique spots detected. An overall of 83 qualitative or quantitative significant differential spots were found among the four methods. Based on the 2- DE gel map, the method is expected to benefit the development of high-level proteomic and biochemical studies of A ndrographis paniculata, which may also be applied to other recalcitrant medicinal plant tissues.
- Subjects
PROTEINS; EXTRACTION (Chemistry); TWO-dimensional electrophoresis; ANTINEOPLASTIC agents; HERBAL medicine; METABOLITES
- Publication
Biotechnology & Applied Biochemistry, 2013, Vol 60, Issue 5, p521
- ISSN
0885-4513
- Publication type
Article
- DOI
10.1002/bab.1126