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- Title
Association of Glutathione with Flowering in Arabidopsis thaliana.
- Authors
Ogawa, Ken'ichi; Tasaka, Yasushi; Mino, Masanobu; Tanaka, Yoshikazu; Iwabuchi, Masaki
- Abstract
In order to study the relationship between GSH and flowering, wild-type and late-flowering mutant, fca-1, of Arabidopsis thaliana were treated with l-buthionine sulfoximine (BSO), a specific inhibitor of GSH biosynthesis, under long-day conditions. BSO treatment of the fca-1 mutant starting at 17 d after imbibition promoted flowering. However, when the treatment was started at 12 d after imbibition, BSO treatment at 10–4 M resulted in an inhibition of flowering. This inhibitory effect of BSO on flowering was abolished by GSH treatment at 10–4 M, although GSH treatment at an increased concentration of 10–3 M clearly delayed flowering. In contrast, BSO treatment of wild-type plants starting at 12 d after imbibition promoted flowering, whose effect was abolished by GSH application. In the fca-1 mutant, whose endogenous GSH levels were high, chilling treatment lowered the GSH levels and promoted flowering, as was the case in the BSO treatment. An A. thaliana mutant, cad2-1, which has a defect in GSH biosynthesis also exhibited late flowering. The late-flowering phenotype of this mutant tended to be strengthened by BSO and abolished by GSH treatment. These results suggest that flowering is associated with the rate of GSH biosynthesis and/or the levels of GSH in A. thaliana.
- Subjects
ARABIDOPSIS thaliana; SULFOXIMINES; GLUTATHIONE; OXIDATION-reduction reaction; BIOSYNTHESIS
- Publication
Plant & Cell Physiology, 2001, Vol 42, Issue 5, p524
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pce065