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- Title
A Pumpkin 72-kDa Membrane Protein of Precursor-Accumulating Vesicles Has Characteristics of a Vacuolar Sorting Receptor.
- Authors
Shimada, Tomoo; Kuroyanagi, Miwa; Nishimura, Mikio; Hara-Nishimura, Ikuko
- Abstract
Precursor-accumulating (PAC) vesicles were previously shown to mediate the transport of the precursor of a major storage protein (pro2S albumin) to protein-storage vacu-oles in developing pumpkin cotyledons. In this study, we characterized two homologous proteins from PAC vesicles, a 72 kDa protein (PV72) and an 82 kDa protein (PV82). PV72 and PV82 showed an ability to bind to peptides derived from both an internal propeptide and a C-terminal peptide of pro2S albumin. PV72 was predicted to be a type I integral membrane protein with epidermal growth factor (EGF)-like motifs. These results suggest that PV72 and PV82 are potential sorting receptors for 2S albumin to protein-storage vacuoles.
- Subjects
PUMPKINS; MEMBRANE proteins; PROTEIN precursors; VESICLES (Cytology); EPIDERMAL growth factor; COTYLEDONS; C-terminal binding proteins
- Publication
Plant & Cell Physiology, 1997, Vol 38, Issue 12, p1414
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/oxfordjournals.pcp.a029138