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- Title
Effect of Qinbai Qingfei Concentrated Pellets on substance P and neutral endopeptidase of rats with post-infectious cough.
- Authors
Jia, Weigang; Wang, Wei; Li, Rui; Zhou, Quanyu; Qu, Ying; Jia, Yumei; Zhang, Zhiheng; Wan, Chengwei; Zhang, Wanwan
- Abstract
Background: In recent years, it has been reported that Qinbai Qingfei Concentrated Pellet (QQCP) has the effect of relieving cough and reducing sputum. However, the therapeutic potentials of QQCP on post-infectious cough (PIC) rat models has not been elucidated. So the current study was aimed to scientifically validate the efficacy of QQCP in post infectious cough. Methods: All rats were exposed to sawdust and cigarette smokes for 10 days, and intratracheal lipopolysaccharide (LPS) and capsaicin aerosols. Rats were treated with QQCP at dose of 80, 160, 320 mg/kg. Cough frequency was monitored twice a day for 10 days after drug administration. Inflammatory cell infiltration was determined by ELISA. Meanwhile, the histopathology of lung tissue and bronchus in rats were evaluated by hematoxylin-eosin staining (H&E). Neurogenetic inflammation were measured by ELISA and qRT-PCR. Results: QQCP dose-dependently decreased the cough frequency and the release of pro-inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8, but exerted the opposite effects on the secretion of anti-inflammatory cytokines IL-10 and IL-13 in BALF and serum of PIC rats. The oxidative burden was effectively ameliorated in QQCP-treated PIC rats as there were declines in Malondialdehyde (MDA) content and increases in Superoxide dismutase (SOD) activity in the serum and lung tissue. In addition, QQCP blocked inflammatory cell infiltration into the lung as evidenced by the reduced number of total leukocytes and the portion of neutrophils in the broncho - alveolar lavage fluid (BALF) as well as the alleviated lung damage. Furthermore, QQCP considerable reversed the neurogenetic inflammation caused by PIC through elevating neutral endopeptidase (NEP) activity and reducing Substance P (SP) and Calcitonin gene related peptide (CGRP) expression in BALF, serum and lung tissue. Conclusions: Our study indicated that QQCP demonstrated a protective role of PIC and may be a potential therapeutic target of PIC.
- Subjects
THERAPEUTIC use of plant extracts; CYTOKINES; INTERLEUKINS; STATISTICS; BRONCHOALVEOLAR lavage; STAINS &; staining (Microscopy); ANIMAL experimentation; ONE-way analysis of variance; NEUROPEPTIDES; INFLAMMATION; SUPEROXIDE dismutase; RATS; MALONDIALDEHYDE; OXIDATIVE stress; COUGH; ENZYME-linked immunosorbent assay; TUMOR necrosis factors; FLUORESCENT antibody technique; DESCRIPTIVE statistics; RESEARCH funding; ENDOPEPTIDASES; PLANT extracts; POLYMERASE chain reaction; DATA analysis software; DATA analysis; CHINESE medicine; POST-infectious disorders
- Publication
BMC Complementary Medicine & Therapies, 2020, Vol 20, Issue 1, pN.PAG
- ISSN
2662-7671
- Publication type
Article
- DOI
10.1186/s12906-020-03081-5