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- Title
Effects of sevoflurane post-conditioning in cerebral ischemia-reperfusion injury via TLR4/NF-κB pathway in rats.
- Authors
SHI, C. -X.; DING, Y. -B.; JIN, J.; LI, T.; MA, J. -H.; QIAO, L. -Y.; PAN, W. -Z.; LI, K. -Z.; YU, F.
- Abstract
OBJECTIVE: The aim of the study was to investigate the anti-inflammatory effect of sevoflurane post-conditioning on cerebral ischemia- reperfusion injury in rats. MATERIALS AND METHODS: Thirty Sprague Dawley (SD) rats were randomly divided into 3 groups: sham operation group (Sham), ischemia/ reperfusion injury (I/R) group and sevoflurane post-conditioning group (Se). Hematoxylin-eosin (HE) staining was used to observe the inflammatory response in the brain tissue. The levels of TNF-α, IL- 1β, IL-6 in serum were measured by ELISA. The mRNA and protein expression of TLR4 and NF-κB p65 were detected by RT-PCR and Western blot in the brain tissue. RESULTS: The post-conditioning of sevoflurane decreased the level of inflammatory reaction in ischemic-reperfusion rat cerebral infarction area and reduced the levels of pro-inflammatory cytokines such as TNF-α, IL-1β, IL-6 in rats with ischemia- reperfusion injury. In addition, after treatment with sevoflurane, the mRNA and protein expression of TLR4 and NF-κBp65 in TLR4/NF-κB pathway was inhibited. CONCLUSIONS: Sevoflurane post-conditioning can decrease the inflammatory reaction in cerebral infarct area induced by cerebral ischemia- reperfusion injury in rats. The neuroprotective effect mechanism of sevoflurane may be related to TLR4/NF-κB signaling pathway.
- Subjects
SEVOFLURANE; ANTI-inflammatory agents; CEREBRAL ischemia; TREATMENT of reperfusion injuries; TOLL-like receptors; NF-kappa B; PREVENTION; THERAPEUTICS
- Publication
European Review for Medical & Pharmacological Sciences, 2018, Vol 22, Issue 6, p1770
- ISSN
1128-3602
- Publication type
Article