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- Title
Induction and isolation of pigmentation mutants of Porphyra yezoensis (Bangiales, Rhodophyta) by heavy-ion beam irradiation.
- Authors
Niwa, Kyosuke; Hayashi, Yoriko; Abe, Tomoko; Aruga, Yusho
- Abstract
The present study describes the isolation of pigmentation mutants of Porphyra yezoensis Ueda induced by heavy-ion beam irradiation for the first time. The gametophytic blades were irradiated with 12C+6 ion beams within a dose range of 25–400 Gy. From the survival rate and cell growth of the irradiated blades, it is suggested that a dose of 150 Gy or less is suitable to induce mutation for the isolation of mutants of P. yezoensis. After irradiation, red, green and deep reddish brown-colored gametophytic blades developed from archeospores that were released from each of the mutated cell clusters of the respective different colors, and the red mutant strain (IBY-R1) and green mutant strain (IBY-G1) were established as a conchocelis colony in culture. Blades of the mutants were characterized by their growth and photosynthetic pigment contents compared with those of the wild-type. From these results, it is clear that heavy-ion beam mutagenesis will be an effective tool for genetic and breeding studies of Porphyra, and also for other algal research.
- Subjects
ION bombardment; RED algae; RADIOGENETICS; GENETIC mutation; BIOLOGICAL variation
- Publication
Phycological Research, 2009, Vol 57, Issue 3, p194
- ISSN
1322-0829
- Publication type
Article
- DOI
10.1111/j.1440-1835.2009.00539.x