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- Title
Selective cytotoxicity of an oxygen-radical-generating enzyme conjugated to a monoclonal antibody.
- Authors
Battelli, Maria Giulia; Abbondanza, Ada; Tazzari, P. L.; Dinota, A.; Rizzi, Simonetta; Grassi, G.; Gobbi, M.; Stirpe, F.
- Abstract
The monoclonal antibody 8A, which recognizes a human plasma cell-associated antigen, was covalently linked to xanthine oxidase in a conjugate maintaining both immunological and enzymatic properties. A significant degree of target cell lysis was obtained at an enzyme concentration that was ineffective on non-target cells and on myeloid staminal cells (CFU-GM). The cytotoxic activity was abolished by an excess of antibody, by allopurinol and by superoxide dismutase and catalase. A possible use of the conjugate for bone marrow purging in multiple myeloma patients is suggested.
- Subjects
MONOCLONAL antibodies; PLASMA cell diseases; IMMUNOLOGIC diseases; XANTHINE oxidase; IMMUNOGLOBULINS; BONE marrow
- Publication
Clinical & Experimental Immunology, 1988, Vol 73, Issue 1, p128
- ISSN
0009-9104
- Publication type
Article