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- Title
Enhanced Transfection of Human Mesenchymal Stem Cells Using a Hyaluronic Acid/Calcium Phosphate Hybrid Gene Delivery System.
- Authors
Lee, Jung Eun; Yin, Yue; Lim, Su Yeon; Kim, E. Seul; Jung, Jaeback; Kim, Dahwun; Park, Ji Won; Lee, Min Sang; Jeong, Ji Hoon
- Abstract
Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.
- Subjects
HUMAN stem cells; MESENCHYMAL stem cells; GENE transfection; CALCIUM phosphate; HYALURONIC acid; BONE morphogenetic proteins
- Publication
Polymers (20734360), 2019, Vol 11, Issue 5, p798
- ISSN
2073-4360
- Publication type
Article
- DOI
10.3390/polym11050798