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- Title
Is the MYH16 still a useful protein in human masticatory muscles?
- Authors
Vermiglio, Giovanna; Cutroneo, Giuseppina; Magaudda, Ludovico; Rizzo, Giuseppina; Centofanti, Antonio; Trimarchi, Fabio; Pergolizzi, Simona
- Abstract
The MYH16 gene encodes the myosin heavy chain protein that plays a key role in mammals' muscles. In details, MYH16 is characteristic of temporalis and masseter muscles of the jaw. This protein is functional in non-human primates and it is correlated to the high power of jaw muscles. In human, MYH16 gene is inactivated and it plays as pseudogene because of a mutation that leads to a very low mRNA transcription level. This low level of mRNA in human seems to be responsible of a small size of jaw muscles if compared to the non-human primates (1). The aim of the present work was to analyse the possible expression of MYH16 protein in human masseter and temporalis muscles of subject with different occlusal characteristics and with different type of malocclusion. We have processed muscle samples for microarray, western blot and immunofluorescence techniques. Results have shown that MYH16 protein is still detectable in human masseter and temporalis muscles and that both mRNA and protein level increase in severe malocclusion. On this basis, the present data show that this protein is still expressed at basal level in human masticatory muscles and that their expression could be influenced by the need of muscle tissue.
- Subjects
MASTICATORY muscles; MASSETER muscle; TEMPORALIS muscle; PROTEINS; MUSCLE strength; PROTEIN expression; HUMAN beings
- Publication
Italian Journal of Anatomy & Embryology / Archivio Italiano di Anatomia Ed Embriologia, 2021, Vol 125, p110
- ISSN
1122-6714
- Publication type
Article