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- Title
Concurrent hypermethylation of multiple tumor-related genes in gastric carcinoma and adjacent normal tissues.
- Authors
Leung, Wai K.; Yu, Jun; Ng, Enders K. W.; To, Ka Fai; Ma, Po K.; Lee, Tin Lap; Go, Minnie Y.Y.; Chung, S. C. Sydney; Sung, Joseph J. Y.; Leung, W K; Yu, J; Ng, E K; To, K F; Ma, P K; Lee, T L; Go, M Y; Chung, S C; Sung, J J
- Abstract
<bold>Background: </bold>Transcriptional silencing by CpG-island hypermethylation now is believed to be an important mechanism of tumorigenesis. To date, studies on CpG-island hypermethylation in gastric carcinoma and adjacent normal tissues are few. <bold>Methods: </bold>The authors examined 5 gastric carcinoma cell lines, 26 frozen gastric carcinoma tissues and their adjacent nontumor area for concurrent CpG-island hypermethylation in 6 tumor-related genes (p15, p16, E-cadherin, GST-pi, hMLH1, and VHL) by methylation-specific polymerase chain reaction. Nontumorous gastric tissues from 10 gastritis patients were used as controls. <bold>Results: </bold>Hypermethylation was not detected in any tissue taken from gastritis patients but was identified in all 5 cell lines and in 24 (92.3%) gastric carcinoma patients. CpG-island methylation in tumor-related genes also was detected in 7 out of the 25 adjacent normal tissues from cancer patients. Hypermethylation of E-cadherin, p15, and p16 were detected more frequently than GST-pi and hMLH1, whereas aberrant methylation of VHL was not detected. Concurrent hypermethylation in 2 or more tumor-related genes was detected in 3 out of the 5 gastric carcinoma cell lines, 22 (84.6%) tumor samples, and 5 (20%) adjacent gastric tissues. Eighteen (69.2%) tumor samples showed hypermethylation in >or= 3 genes. <bold>Conclusions: </bold>The current study showed that concurrent hypermethylation of multiple tumor-related genes is detected frequently in gastric carcinoma and adjacent normal tissues. Study findings suggested that a mechanism that leads to dysregulation in CpG-island methylation is likely to be involved in the early gastric carcinogenesis process.
- Subjects
PROTEINS; STOMACH tumors; CANCER cell culture; ONCOGENES; IMMUNOHISTOCHEMISTRY; NUCLEAR proteins; CELL cycle proteins; CANCER; GLYCOPROTEINS; METHYLATION; TRANSCRIPTION factors; POLYMERASE chain reaction; CARRIER proteins
- Publication
Cancer (0008543X), 2001, Vol 91, Issue 12, p2294
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(20010615)91:12<2294::AID-CNCR1261>3.0.CO;2-G