We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Methylene Blue Treatment of Grafts During Cold Ischemia Time Reduces the Risk of Hepatitis C Virus Transmission.
- Authors
Helfritz, Fabian A; Bojkova, Denisa; Wanders, Verena; Kuklinski, Nina; Westhaus, Sandra; Horn, Charlotte von; Rauen, Ursula; Gallinat, Anja; Baba, Hideo A; Skyschally, Andreas; von Horn, Charlotte; Swoboda, Sandra; Kinast, Volker; Steinmann, Eike; Heusch, Gerd; Minor, Thomas; Meuleman, Philip; Paul, Andreas; Ciesek, Sandra
- Abstract
<bold>Background: </bold>Although organ shortage is a rising problem, organs from hepatitis C virus (HCV) ribonucleic acid (RNA)-positive donors are not routinely transplanted in HCV-negative individuals. Because HCV only infects hepatocytes, other organs such as kidneys are merely contaminated with HCV via the blood. In this study, we established a protocol to reduce HCV virions during the cold ischemic time.<bold>Methods: </bold>Standard virological assays were used to investigate the effect of antivirals, including methylene blue (MB), in different preservation solutions. Kidneys from mini pigs were contaminated with Jc1 or HCV RNA-positive human serum. Afterwards, organs were flushed with MB. Hypothermic machine perfusion was used to optimize reduction of HCV.<bold>Results: </bold>Three different antivirals were investigated for their ability to inactivate HCV in vitro. Only MB completely inactivated HCV in the presence of all perfusion solutions. Hepatitis C virus-contaminated kidneys from mini pigs were treated with MB and hypothermic machine perfusion without any negative effect on the graft. Human liver-uPA-SCID mice did not establish HCV infection after inoculation with flow through from these kidneys.<bold>Conclusions: </bold>This proof-of-concept study is a first step to reduce transmission of infectious HCV particles in the transplant setting and might serve as a model for other relevant pathogens.
- Subjects
ANTIVIRAL agents; RNA; METHYLENE blue; HEPATITIS C virus; PATHOGENIC microorganisms
- Publication
Journal of Infectious Diseases, 2018, Vol 218, Issue 11, p1711
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiy386