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- Title
IGF-1 reduces the apoptosis of endothelial progenitor cells induced by oxidized low-density lipoprotein by the suppressing caspasse-3 activity.
- Authors
Yonggang Wu; Qiru Wang; Lamei Cheng; Jian Wang; Xuan Sun; Guangxiu Lu
- Abstract
Dyslipidemia increase the risk for coronary heart disease in part by impairing endothelial integrity. Endothelial progenitor cells(EPCs) play a pivotal role in reendotheliazation. In previous study, oxidized low-density lipoprotein (oxLDL) affects the apoptosis of the EPCs. However, the precise mechanism is still unclear. Therefore, we focused in this study to understand whether oxLDL can induce caspase-3 activity and insulin like growth factor-1(IGF-1) can prevent the EPC by the decrease the caspase-3 activity and the apoptosis in the EPCs. EPCs were isolated from peripheral blood and characterized. The EPCs were cultured with IGF-1 for 30 min and then were treated with oxLDL for 48 h. The apoptosis of the Endothelial progenitor cells was checked by the flow cytometry and, while caspase-3 activity was measured simultaneously. After the EPC were treated, the mean of apoptotic rates and caspase-3 enzyme activity significantly decreased. in EPCs treated with IGF-1, the decrease of the caspase-3 activity by oxLDL was blocked and apoptosis prevented. Therefore, IGF-1 can reduced the apoptosis of endothelial progenitor cells induced by oxLDL. This effect may be mediated by significantly suppressing caspase-3 activity.
- Subjects
SOMATOMEDIN; APOPTOSIS; VASCULAR endothelium; LIPOPROTEINS; CORONARY heart disease risk factors
- Publication
Cell Research, 2008, Vol 18, pS159
- ISSN
1001-0602
- Publication type
Abstract
- DOI
10.1038/cr.2008.249