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- Title
Regulatory mechanism of lncRNA miR143HG in miR-504 and its effect on proliferation and apoptosis of non-small cell lung cancer cells.
- Authors
Zhe Li; Jinhua Liu; Yingqun Zhu; Qian Cai
- Abstract
Purpose: To investigate the expression and functional role of miR143HG in non-small cell lung cancer (NSCLC), and its effect on human lung adenocarcinoma cell behavior. Methods: Differential expression of miR143HG between NSCLC tissues and healthy counterparts was identified through bioinformatic analysis. Subsequently, this expression difference in A549 and BEAS- 2B cells was validated using quantitative polymerase chain reaction (qPCR). Overexpression of miR143HG in A549 cells was achieved through liposome-mediated transfection, while cell proliferation as well as apoptosis levels were assessed using cell counting kit-8 (CCK-8) assay, flow cytometry, and protein blotting. Expression of miR-504 (predicted as a target of miR143HG) was determined. Furthermore, A549 cells that overexpress both miR143HG and miR-504 were generated for comparison with cells overexpressing only miR143HG. Results: Compared to BEAS-2B cells, A549 cells showed significantly reduced miR143HG and elevated miR-504 levels (p < 0.05). In A549 cells with miR143HG overexpression, cell proliferation and miR-504 expression significantly reduced while pro-apoptotic proteins (BAX, p53) significantly increased (p < 0.05). Simultaneous overexpression of miR143HG and miR-504 in A549 cells counteracted the effect of miR143HG alone, thus enhancing proliferation and diminishing pro-apoptotic protein expression, similar to control. Conclusion: Downregulation of MiR143HG occurs in human lung adenocarcinoma cells. Upregulation of MiR143HG impedes A549 cell proliferation, promotes apoptosis through pro-apoptotic protein modulation and suppresses miR-504. Co-overexpression of miR143HG and miR-504 reverses these effects, resembling control conditions. Thus, miR143HG exerts its anti-cancer effect in A549 cells by modulating miR-504 and activating p53 pathway.
- Subjects
NON-small-cell lung carcinoma; CANCER cell proliferation; WESTERN immunoblotting; POLYMERASE chain reaction; CELL proliferation
- Publication
Tropical Journal of Pharmaceutical Research, 2024, Vol 23, Issue 8, p1231
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v23i8.1