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- Title
Genetic and Pathogenic Characterization of an Iridovirus from the Cultured Largemouth Bass Micropterus salmoides.
- Authors
Zhang, Yi-Fan; Li, Ying; Li, Peng-Tian; Jiang, Jing; Zeng, Wei-Hang; Ye, Kun; Wang, Yi-Lei; Zou, Peng-Fei
- Abstract
The largemouth bass is a freshwater aquacultured fish species of great economic importance in China. With the rapid development of aquaculture industry and the increase in the aquaculture density of the fish, various infectious pathogens, including parasites, bacteria, and viruses, have been widely spread, which have caused huge losses to the aquaculture industry. Among them, largemouth bass iridovirus (LMBV) is one of the most harmful pathogens. In the present study, a virus strain named LMBV-GDSD was isolated from cultured largemouth bass and was successfully proliferated in FHM and EPC cells, with numerous viral particles observed in the infected cells under transmission electron microscopy analysis. The annotated complete genome of LMBV-GDSD was 99,285 bp and contained 102 ORFs. Based on genomic sequence alignment and phylogenetic analysis, the identified LMBV-GDSD belonged to the genus Ranavirus of Iridoviridae and was pathogenic to largemouth bass under regression infection experiments. In addition, the infection of LMBV-GDSD in largemouth bass could significantly up-regulate the expression of antiviral immune-related genes such as IRF3, IRF7, and Mx. It is thus providing valuable genetic data for a deeper understanding of the pathogenic mechanism of iridovirus in largemouth bass.
- Subjects
CHINA; FRESHWATER fishes; LARGEMOUTH bass; FISH farming; TRANSMISSION electron microscopy; AQUACULTURE industry; SEQUENCE alignment
- Publication
Fishes (MDPI AG), 2024, Vol 9, Issue 8, p314
- ISSN
2410-3888
- Publication type
Article
- DOI
10.3390/fishes9080314