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- Title
Effects of Recombinant Human AMH on SCF Expression in Human Granulosa Cells.
- Authors
Hu, Rong; Lou, Yan; Wang, Fei-Miao; Ma, Hui-Ming; Wu, Xing; Zhang, Xiao-Mei; Li, Juan; Sun, Ying-Pu
- Abstract
The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group ( p < 0.05). We further showed that rhAMH inhibited SCF expression at mRNA and protein levels.
- Subjects
ANTI-Mullerian hormone; STEM cell factor; GRANULOSA cells; MESSENGER RNA; POLYMERASE chain reaction; ENZYME-linked immunosorbent assay; IMMUNOFLUORESCENCE
- Publication
Cell Biochemistry & Biophysics, 2013, Vol 67, Issue 3, p1481
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-013-9649-x