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- Title
Spontaneous single nucleotide polymorphism in porcine microRNA-378 seed region leads to functional alteration.
- Authors
chai, Jie; Chen, Lei; Luo, Zonggang; Zhang, Tinghuan; Chen, Li; Lou, Pengbo; Sun, Wenyang; Long, Xi; Lan, Jing; Wang, Jinyong; Pu, Hongzhou; Qiu, Jinjie; Shuai, Surong; Guo, Zongyi
- Abstract
Sequence variation in a microRNA (miRNA) seed region can influence its biogenesis and effects on target mRNAs; however, in mammals, few seed region mutations leading to functional alterations have been reported to date. Here, we report the identification of a single nucleotide polymorphism (SNP) with functional consequence located in the seed region of porcine miR-378. In vitro analysis of this rs331295049 A17G SNP showed significantly up-regulated expression of the mature miR-378 (miR-378/G). In silico target prediction indicated that the SNP would modulate secondary structure and result in functional loss affecting >85% of the known target genes of the wild-type miR-378 (miR-378/A), and functional gain affecting >700 new target genes, and dual-luciferase reporter assay verified this result. This report of a SNP in the seed region of miR-378 leads to functional alteration and indicates the potential for substantive functional consequences to the molecular physiology of a mammalian organism.
- Subjects
SINGLE nucleotide polymorphisms; MICRORNA; CRISPRS
- Publication
Bioscience, Biotechnology & Biochemistry, 2018, Vol 82, Issue 7, p1081
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2018.1459175