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- Title
Modulation of the E. coli rpoH Temperature Sensor with Triptycene- Based Small Molecules.
- Authors
Barros, Stephanie A.; Yoon, Ina; Chenoweth, David M.
- Abstract
Regulation of the heat shock response (HSR) is essential in all living systems. In E. coli, the HSR is regulated by an alternative s factor, σ32, which is encoded by the rpoH gene. The mRNA of rpoH adopts a complex secondary structure that is critical for the proper translation of the σ32 protein. At low temperatures, the rpoH gene transcript forms a highly structured mRNA containing several three-way junctions, including a rare perfectly paired three-way junction (3WJ). This complex secondary structure serves as a primitive but highly effective strategy for the thermal control of gene expression. In this work, the first small-molecule modulators of the E. coli σ32 mRNA temperature sensor are reported.
- Subjects
ESCHERICHIA coli; BACTERIAL genes; TEMPERATURE sensors; SMALL molecules; TRIPTYCENES; GENETIC transcription in bacteria
- Publication
Angewandte Chemie, 2016, Vol 128, Issue 29, p8398
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201601626