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- Title
Binding of Chromium(III) to Transferrin Could Be Involved in Detoxification of Dietary Chromium(III) Rather than Transport of an Essential Trace Element.
- Authors
Levina, Aviva; Pham, T. H. Nguyen; Lay, Peter A.
- Abstract
CrIII binding to transferrin (Tf; the main FeIII transport protein) has been postulated to mediate cellular uptake of CrIII to facilitate a purported essential role for this element. Experiments using HepG2 (human hepatoma) cells, which were chosen because of high levels of the transferrin receptor, showed that CrIII binding to vacant FeIII-binding sites of human Tf effectively blocks cellular CrIII uptake. Through bio-layer interferometry studies of the Tf cycle, it was found that both exclusion and efflux of Cr2IIITf from cells was caused by 1) relatively low Cr2Tf affinity to cell-surface Tf receptors compared to Fe2Tf, and 2) disruption of metal release under endosomal conditions and post-endosomal Tf dissociation from the receptor. These data support mounting evidence that CrIII is not essential and that Tf binding is likely to be a natural protective mechanism against the toxicity and potential genotoxicity of dietary Cr through blocking CrIII cellular accumulation.
- Subjects
METALLIC bonds; TRANSFERRIN; CHROMIUM; HOMEOSTASIS; METAL toxicology; TRACE elements
- Publication
Angewandte Chemie, 2016, Vol 128, Issue 28, p8236
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/anie.201602996