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- Title
Reversibly Triggered Protein-Ligand Assemblies in Giant Vesicles.
- Authors
Peters, Ruud J. R. W.; Nijemeisland, Marlies; van Hest, Jan C. M.
- Abstract
External small-molecule triggers were used to reversibly control dynamic protein-ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small-molecule substrates, thereby modulating the pH-sensitive interaction between a Ni-NTA ligand on the vesicle membrane and an oligohistidine-tagged protein in the lumen. By alternating the small-molecule substrates the interaction could be reversed.
- Subjects
VESICLES (Cytology); PROTEIN binding; LIGAND binding (Biochemistry); SMALL molecules; ALCOHOL dehydrogenase; HYDROGEN-ion concentration; HISTIDINE
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 33, p9750
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201502920