We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Live-Cell Imaging of Endogenous mRNAs with a Small Molecule.
- Authors
Sato, Shin-ichi; Watanabe, Mizuki; Katsuda, Yousuke; Murata, Asako; Wang, Dan Ohtan; Uesugi, Motonari
- Abstract
Determination of subcellular localization and dynamics of mRNA is increasingly important to understanding gene expression. A new convenient and versatile method is reported that permits spatiotemporal imaging of specific non-engineered RNAs in living cells. The method uses transfection of a plasmid encoding a gene-specific RNA aptamer, combined with a cell-permeable synthetic small molecule, the fluorescence of which is restored only when the RNA aptamer hybridizes with its cognitive mRNA. The method was validated by live-cell imaging of the endogenous mRNA of β-actin. Application of the technology to mRNAs of a total of 84 human cytoskeletal genes allowed us to observe cellular dynamics of several endogenous mRNAs including arfaptin-2, cortactin, and cytoplasmic FMR1-interacting protein 2. The RNA-imaging technology and its further optimization might permit live-cell imaging of any RNA molecules.
- Subjects
CELL imaging; GENE expression; MESSENGER RNA; SPATIOTEMPORAL processes; GREEN fluorescent protein; IN situ hybridization; FLUORESCENCE microscopy
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 6, p1875
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201410339