We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Enhancement of the catalytic activity of an artificial phosphotriesterase using a molecular imprinting technique.
- Authors
Zihui Meng; Yamazaki, Tomohiko; Sode, Koji
- Abstract
An artificial phosphotriesterase (PTE) was constructed by co-polymerization of 4(5)-vinylimidazole-Zn2+-methacrylic acid cluster with a divinylbenzene polymer. Compared with the spontaneous hydrolysis, the resulting polymer catalyst caused 105-fold rate acceleration towards the hydrolysis of diethyl p-nitrophenyl phosphate (Paraoxon). The catalytic activity of the polymer catalyst could be enhanced for 30% using molecular imprinting technique and the molecularly-imprinted catalyst (MIC) showed a turnover rate of 7.4×10-2 s-1 towards the hydrolysis of Paraoxon. The MIC also hydrolyzed thiophosphates and phosphorothiolate triester pesticides. Construction of an amperometric sensor employing the MIC as catalyst achieved a detection limit of 0.1 mM Paraoxon.
- Subjects
MOLECULAR imprinting; POLYMERIZATION; METHACRYLIC acid; STYRENE; HYDROLYSIS; CATALYSIS
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 13, p1075
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1024152229526