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- Title
Polysaccharide-enriched fraction isolated from Duchesnea chrysantha protects against oxidative damage.
- Authors
Kim, In Gyu; Jung, Il Lae; Oh, Tae Jeong; Kim, Kug Chan; Shim, Hae Won
- Abstract
Reactive oxygen species (ROS)-related oxidative damages have been implicated in a wide variety of the pathological processes such as atherosclerosis, inflammation and carcinogenesis. A polysaccharide-enriched fraction (PEF) was isolated from Duchesnea chrysantha, a herbaceous plant, and its antioxidant activity was demonstrated using several assay systems in vitro. The PEF effectively inhibited Cu2+-stimulated low density lipoprotein oxidation in a concentration-dependent way and retarded the conjugated diene formation with the enhancement of the lag phase during oxidation. An assay for DNA strand breaks showed that PEF strongly protected DNA against damage caused by UV or by ⋅OH or ⋅O2- generated in the metal-catalyzed oxidation system. PEF effectively inhibited Nitroblue Tetrazolium reduction mediated by ⋅O2- in a dose-dependent manner. It also competed with 2-deoxy-d-ribose in absorbing ⋅OH generated by γ-irradiation (600 Gy) and thus inhibited the formation malondialdehyde. In conclusion, these evidences indicate that PEF may act as an antioxidant to scavenge ⋅O2-, ⋅OH or LO2⋅ directly. Our findings suggest the possibility that it may play a role as a potential therapeutic antioxidant in treatment of oxidative damage-derived diseases.
- Subjects
REACTIVE oxygen species; POLYSACCHARIDES; MALONDIALDEHYDE; LOW density lipoproteins; PROTEIN fractionation; ATHEROSCLEROSIS; INFLAMMATORY mediators; MOLECULAR carcinogenesis; BIOREMEDIATION
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 16, p1299
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1019812202099