We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Downstream separation of poly(hydroxyalkanoates) using crude enzyme consortia produced via solid state fermentation integrated in a biorefinery concept.
- Authors
Kachrimanidou, Vasiliki; Kopsahelis, Nifeolaos; Vlysidis, Anestis; Papanikolaou, Seraphim; Kookos, Ioannis K.; Martínez, Belén Monje; Escrig Rondánd, Maria Consuelo; Koutinas, Apostolis A.
- Abstract
Crude enzyme produced via solid state fermentation using the fungal strain Aspergillus oryzae was used to lyse cells of Cupriavidus necator, enabling the recovery of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-12mol% 3HV). A central composite design was employed in order to optimize the temperature and the pH value leading to the highest lysis of Cupriavidus necator cells (88.9 ± 0.4%) and the optimal hydrolysis of protein contained in bacterial cells. Enzymatic lysis of bacterial cells was also carried out at the optimum temperature and uncontrolled pH value leading to P(3HB-co-12mol% 3HV) recovery yield and purity of 98% and 96.7%, respectively. The bacterial cell lysate obtained after the separation of P(3HB-co-12mol% 3HV) granules was evaluated as nutrient-rich supplement together with crude glycerol for the production of poly(3-hydroxybutyrate) in shake flask cultures. This novel downstream separation process could be integrated in a sunflower-based biorefinery reducing the cost of poly(hydroxyalkanoate) recovery and fermentation medium formulation.
- Subjects
BIOCHEMICAL engineering; SOLID-state fermentation; KOJI; POLYHYDROXYALKANOATES; TEMPERATURE effect; DIETARY supplements
- Publication
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2016, Vol 100, Issue Part A, p323
- ISSN
0960-3085
- Publication type
Article
- DOI
10.1016/j.fbp.2016.08.002