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- Title
Purification and partial characterization of antifreeze proteins from leaves of Ligustrum lucidum Ait.
- Authors
Yujie Cai; Shang Liu; Xiangru Liao; Yanrul Ding; Jun Sun; Dabing Zhang
- Abstract
Three AFPs (AFP-1, AFP-2, APP-3) of Ligustrum lucidum Ait leaves were purified by using ice-affinity, chromatogra- phy separation on a DEAE-cellulose-32 column and a Sephadex G100 column. The ice-affinity proteins were about 1.2 mg/100 g leaves. Their molecular weight was 66.1, 26.3, and 20.2 kDa, respectively. Their thermal hysteresis activity was 0.379, 0.678, and 0.460°C respectively. Asx was very abundant in these AFPs; its molar ratio was 22.2, 24.9, and 27.8%, respectively. AFP-2 was effective to protect peroxidase, β-glucosidase, and trehalose synthase from freeze-thawing process. Cryoprotection of AFP-2 is better than trehalose.
- Subjects
PRIVETS; CHROMATOGRAPHIC analysis; CELLULOSE; SEPHADEX; PEROXIDASE
- Publication
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2011, Vol 89, Issue 2, p98
- ISSN
0960-3085
- Publication type
Article
- DOI
10.1016/j.fbp.2010.04.002