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- Title
In vivo regeneration of bladder muscular wall using decellularized colon matrix: an experimental study.
- Authors
Kajbafzadeh, Abdol-Mohammad; Khorramirouz, Reza; Sabetkish, Shabnam; Ataei Talebi, Mahba; Akbarzadeh, Aram; Keihani, Sorena
- Abstract
<bold>Background: </bold>Finding a proper scaffold for augmentation is a serious challenge in bladder tissue engineering. We hereby aimed to determine the histological aspects of a decellularized colon graft for bladder augmentation in healthy rats.<bold>Methods: </bold>Rat colon tissues were decellularized using perfusion-based method. After partial cystectomy, bladders were grafted with a patch of decellularized colon. Bladder specimens were investigated in 12 rats at 1, 3, and 9 months postoperatively for further histological changes and immunohistochemistry analyses were also performed.<bold>Results: </bold>One month after implantation, partial seeding of new cells was observed. After 3 months continuity of transitional epithelium of natural bladder on the decellularized grafted colon tissue was confirmed with histological and immunohistochemical examinations. All augmented bladders demonstrated a spherical shape without stone formation, necrosis or graft rejection. The presence of urothelium with similar morphology to the natural urothelium and visible blood vessels were found within 3 months of operation. All immunohistochemical markers (except markers of colonic stem cells) were expressed in biopsies obtained 3 months after surgery demonstrating a progressive vascular and smooth muscle cell infiltration into the graft after implantation.<bold>Conclusion: </bold>This study suggests that decellularized colon may provide a viable material for bladder augmentation in rats to pave the road for future applications of this natural collagen scaffold.
- Subjects
CYSTOTOMY; REGENERATIVE medicine; CYSTECTOMY; TISSUE scaffolds; TISSUE engineering; IMMUNOHISTOCHEMISTRY; SMOOTH muscle physiology; ANIMAL experimentation; BIOLOGICAL models; COLON (Anatomy); EPITHELIUM; UROLOGICAL surgery; GUIDED tissue regeneration; RATS; SMOOTH muscle
- Publication
Pediatric Surgery International, 2016, Vol 32, Issue 6, p615
- ISSN
0179-0358
- Publication type
journal article
- DOI
10.1007/s00383-016-3871-8