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- Title
Atypical hyperplasia in mice caused by Mucor irregularis and Rhizopus oryzae isolated from lethal midline granuloma.
- Authors
GE Jie; ZHOU Ya-Bin; LI Dong-Ming
- Abstract
Rhino-orbital-cerebral mycosis and lethal midline granuloma (LMG) share the same clinical manifestations of destructive ulcers occurring in the midline face. Sometimes, LMG can be combined with fungal infections, which are often considered secondary. In our previous case studies, Mucor irregularis PUTH 10050641 and Rhizopus oryzae PUTH 20111112 were isolated from the LMG tissue, and the patients were cured by antifungal therapy. We therefore speculated on that M. irregularis and R. oryzae may be involved in inducing atypical hyperplasia and expression of CD-56 and Ki-67 antigens. In order to verify our inference, mouse skin infection model was made using immunosuppressed ICR mice hypodermically injected with cyclophosphamide and M. irregularis PUTH 10050641 and R. oryzae PUTH 20111112 for observation of pathological changers. Skin specimens obtained were then cultured and histological analysis was made in 1, 2, 3, 4 and 5 weeks after inoculation. Inflammation expression models in different groups were analyzed. The result was that M. irregularis and R. oryzae successfully infected immunocompetent and immunosuppressed ICR mice. The thin-walled hyphae of the mucoralean fungi might be atypical that could not been recognized but could be cultured in media. Pleomorphic cell infiltration could be seen in the infected site, in which atypical cells could be seen. Angioinvasive phenomenon could be seen in the mouse models. A large number of mixed inflammatory cells could be seen pathologically and the proliferating cells gathered around fungal structure. The IOD of Ki-67 and CD-56 was significantly higher in the experimental groups than that in the control ones. Our preliminary studies have shown that M. irregularis and R. oryzae are involved in inducing atypical hyperplasia and NK cell aggregation.
- Subjects
RHIZOPUS oryzae; MUCOR; PYRICULARIA oryzae; GRANULOMA; KI-67 antigen; HYPERPLASIA; RICE blast disease; PHYTOPATHOGENIC fungi
- Publication
Mycosystema, 2019, Vol 38, Issue 8, p1314
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.190166