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- Title
砧木及抗性马尾松松脂组分对松材线虫的响应.
- Authors
李文华; 刘青华; 周志春; 高 凯; 骆定会
- Abstract
[Objective] By inoculating Pinus massoniana Lamb. with pine wood nematode, the changes of pine resin components before and after inoculation were analyzed, so as to provide a theoretical basis for the study of resistance of P. massoniana to pine wood nematode disease. [Method] The experimental material were 5-year-old clones of P. massoniana in Linhai City, Zhejiang Province. We extracted the pine resin components of P. massoniana rootstocks and P. elliottii rootstocks, highly resistant and susceptible P. massoniana inoculated with pine wood nematode for 1, 7 and 15 days and also analyzed the content and dynamic changes of each pine resin component. The effective terpenes were used to treat pine wood nematode. Then we analyzed the survival rate of pine wood nematodes. [Result] The results showed that 19 oleoresin terpenoids were Identified. The abundant terpenoids were α-pinene, β-pinene, carylene, pimaric acid, sandaracopimaric acid, palustric acid/levopimaric acid, dehydroabietic acid, neoabietic acid and abietic acid. In the rootstock of P. massoniana, the content of β-myrcene was significantly higher than that of P. elliottii rootstock 1day after inoculating with pine wood nematode(p<0.05); the contents of the above components had no significant difference among different rootstocks after inoculating with pine wood nematode 7 days and 15 days. The contents of α-pinene, β-pinene, β-laurene, limonene, borneol, longifolene, α-caryophyllene, trans-β-farnesene and neoabietic acid in different resistant P. massoniana changed differently after inoculation with pine wood nematode. In the high resistance P. massoniana, the contents of αpinene and β-pinene were significantly higher than those of susceptible P. massoniana 1 day after inoculating with pine wood nematode(p<0.05); In the high resistance P. massoniana, the content of α-pinene, βpinene, limonene and longifolene were significantly higher than those of susceptible P. massoniana 7 days after inoculating with pine wood nematode(p<0.05); The situation was the same when pine wood nematode was inoculated for 15 days as when pine wood nematode was inoculated for 7 days. The result of gradient concentration test of the above terpenoids showed that: when the concentration of α-pinene was 150 mg·g−1 for 0.5 h, the survival rate of pine wood nematode was 20%. Different concentrations of βpinene solution had the same inhibitory effect on pine wood nematode, and the survival rate reached about 50% when treated for 0.5h. When limonene solution concentration was 10mg·g−1 for 0.5 h, the survival rate of inhibited pine wood nematode was almost zero. When the concentration of longifolene was 10mg·g−1 for 0.5 h, the survival rate of pine wood nematode was 18%. [Conclusion] There are no obvious relationships between different rootstocks and resistance to P. massoniana. There are significant differences in αpinene, β-pinene, limonene and longifolene contents between high and susceptible P. massoniana inoculated with pine wood nematode, and the content of high resistance P. massoniana was significantly higher than those of susceptible P. massoniana. At low concentrations α-pinene, β-pinene, limonene and longifolene inhibit the activity of pine wood nematodes, which shows the four terpenoids are related to resistance of P. massoniana.
- Subjects
ZHEJIANG Sheng (China); PINEWOOD nematode; ABIETIC acid; CONCENTRATION gradient; SURVIVAL rate; LIMONENE; NEMATOCIDES; SWEET cherry
- Publication
Forest Research, 2023, Vol 36, Issue 4, p1
- ISSN
1001-1498
- Publication type
Article
- DOI
10.12403/j.1001-1498.20220419