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- Title
园林废弃物木质素降解真菌的筛选、鉴别及其能力研究.
- Authors
康 跃; 李素艳; 孙向阳; 龚小强; 余克非; 蔡琳琳; 王 琳
- Abstract
[Objective] To obtain a fungus that can efficiently degrade landscaping waste, to study its ability to decomposite various landscaping waste, and to select the fungus strain from the composted landscaping waste by a series of isolation and culture. [Method] The species of lignin decomposition strain were identified by morphological analysis, ITS molecular sequence analysis and phylogenetic tree. Solid-state fermentation experiments were carried out for studying the changes of enzyme activity of laccase (Lac) and manganese peroxidase (MnP) of the strain and their ability to decompose lignin in landscaping waste. [Result] A total of 18 fungus were selected from the composted landscaping waste and a highly efficient lignin-decomposing fungus was selected. The strain was designated as strain No.11, and was identified as Aspergillus nidulans (Eidam) G. Winter by morphological analysis and ITS molecular sequence analysis. The enzyme activity peaks of laccase and manganese peroxidase in solid canisters were determined using Japanese spindle leaves, Japanese spindle branches, juniper leaves, juniper branches and forsythia as the substrates were 388.7 and 138.3, 326.3 and 121.1, 461.4 and 104.6, 342.7 and 128.6, 588.5 and 73.3 U·L-1. The addition of strain 11 increased the lignin decomposition rate of Japanese spindle leaves, Japanese spindle branches, juniper leaves, juniper branches and forsythia after 40 days, respectively, by 20.40%, 22.44%, 29.90%, 25.28%, and 15.77%. [Conclusion] The lignin-decomposing fungus, Aspergillus nidulans (Eidam) G. Winter, has a strong ability to decompose the lignin in the branches and leaves of Japanese spindle, juniper and forsythia common to the landscaping waste in Beijing. Aspergillus nidulans (Eidam) G. Winter may have advantages in decomposing landscaping waste.
- Subjects
BEIJING (China); ASPERGILLUS nidulans; MANGANESE peroxidase; SOLID-state fermentation; SEQUENCE analysis; LACCASE; JUNIPERS
- Publication
Forest Research, 2019, Vol 32, Issue 3, p80
- ISSN
1001-1498
- Publication type
Article
- DOI
10.13275/j.cnki.lykxyj.2019.03.011