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- Title
Immobilization of lipase on the surface of a micro tube and continuous transesterification.
- Authors
Yoshimura, Yuko; Saito, Akane; Mori, Megumi; Osaki, Shusuke; Miyazaki, Takashi; Mori, Hajime
- Abstract
<italic>Candida antarctica</italic> lipase B (CALB) was cross-linked efficiently, using a reagent containing glutaraldehyde (GA), paraformaldehyde (PA), and a polylysine reagent. For the reaction under micro-flow conditions, CALB was immobilized on the inner surface of a polytetrafluoroethylene (PTFE) tube, by flowing the cross-linking reagents in an appropriate flow rate. The immobilized CALB formed a membrane-like structure on the inner surface of the tube and showed catalytic activity for transesterification. Although the enzyme activity was not high at the immobilized CALB, the productivity of micro-flow reaction using immobilized CALB was higher than that of batch-wise reaction using free CALB.Graphical Abstract<graphic></graphic>
- Subjects
LIPASE regulation; ENCAPSULATION (Catalysis); CONTINUOUS flow reactors; TRANSESTERIFICATION; ENZYME activation
- Publication
Journal of Flow Chemistry, 2018, Vol 8, Issue 1, p45
- ISSN
2062-249X
- Publication type
Article
- DOI
10.1007/s41981-018-0006-5