We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
光纤成像技术记录小鼠眶额皮层奖赏相关神经元活性的应用.
- Authors
刘敏; 孙晓红; 杨子成; 杜庚; 雷慧萌
- Abstract
Objective: To investigate the feasibility of fiber photometry technology in recording the activity changes of reward-related neurons in the orbital frontal cortex of mice. Methods: The fiber photometry method was used creatively to record the activity of reward-related neuronal that expressed calcium fluorescent probe(GCaMP6m) in orbital frontal cortex of freely moving mice which were stimulated by drinking syrup. Firstly, adeno-associated virus carrying GCaMP6m were injected into the OFC of postanesthetic mice precisely. Then, one pre-made fiber-optic ceramic ferrule was implanted into the corresponding site carefully,accurately and suitably. The mice that undergone mentioned surgery needed to regain them health and the injected viruses needed to express GCaMP6m efficiently in two weeks. In the second place, the mice that recovered health after surgery were given water-deprivation treatment which sustained 36 hours before recorded reward-related neuronal signal. The reaction activity of frontal cortex pyramidal neurons in mice that stimulated by syrup was recorded by means of fiber photometry system dynamically. In the end,the date that reflected the activity of recorded reward-related neuro in mice’s OFC were analyzed scrupulously using matlab software. At the same time, the anaesthetic mice were subjected to heart perfusion, brain taken, frozen section of removed brain and fluorescence imaging using fluorescence microscope orderly and feelinglessly. The purpose of above complex operations were to affirm the validity of neuronal signal recording site and the level of injected viruese expression. Results: The variation of neuronal activity which associated with reward in OFC of syrup stimulated mice was recorded successfully and clearly using fiber photometry method. The results of data analysis that reflected changed neuronal activity were shown through a heat map and an event-related average plot scientifically.Meantime, the frozen section of mice brain and fluorescence imaging results all demonstrably indicated that the neuronal signal recorded sites were undoubted correct and the expression level of viruses were very high in correspond region. Conclusions: Fiber photometry was able to be used to measure the activity of reward-related neurons in the orbito frontal cortex of free-moving mice when they were given sugar water.
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 8, p1420
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.08.005