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- Title
Improved Properties of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Produced by Comamonas sp. EB172 Utilizing Volatile Fatty Acids by Regulating the Nitrogen Source.
- Authors
Zakaria, Mohd Rafein; Ariffin, Hidayah; Abd-Aziz, Suraini; Hassan, Mohd Ali; Yoshihito Shirai
- Abstract
This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3- hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5,15,25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (Mn) of P(3HB-co-3HV) copolymer reached the highest at 838 x 10³ Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6 - 8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials.
- Publication
BioMed Research International, 2013, Vol 2013, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2013/237806