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- Title
A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing.
- Authors
Kangquan Yin; Ting Han; Guang Liu; Tianyuan Chen; Ying Wang; L. Yu, Alice Yunzi; Yule Liu
- Abstract
CRISPR/Cas has emerged as potent genome editing technology and has successfully been applied in many organisms, including several plant species. However, delivery of genome editing reagents remains a challenge in plants. Here, we report a virus-based guide RNA (gRNA) delivery system for CRISPR/Cas9 mediated plant genome editing (VIGE) that can be used to precisely target genome locations and cause mutations. VIGE is performed by using a modified Cabbage Leaf Curl virus (CaLCuV) vector to express gRNAs in stable transgenic plants expressing Cas9. DNA sequencing confirmed VIGE of endogenous NbPDS3 and NbIspH genes in non-inoculated leaves because CaLCuV can infect plants systemically. Moreover, VIGE of NbPDS3 and NbIspH in newly developed leaves caused photo-bleached phenotype. These results demonstrate that geminivirus-based VIGE could be a powerful tool in plant genome editing.
- Subjects
RNA; PLANT genomes; GENOME editing; RNA editing; NUCLEOTIDE sequencing; TRANSGENIC plants
- Publication
Scientific Reports, 2015, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep14926