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- Title
Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids.
- Authors
Buks, Ralfs; Alnabulsi, Abdo; Zindrili, Rodanthi; Alnabulsi, Ayham; Wang, Alex; Wang, Tiehui; Martin, Samuel A. M.
- Abstract
Serum amyloid A (SAA) proteins belong to a family of acute-phase reactants, playing an integral role in defending the organism from pathological damage. Despite a wealth of data on the regulation of SAA transcripts in teleosts, there is only limited information on these proteins' abundance in fish. The aim of this study is to characterise SAA protein levels in salmonids using a newly developed antibody specific to salmonid SAA. The salmonid SAA antibody detected SAA and accurately discriminated between stimulated and control specimens from rainbow trout macrophage cell line (RTS-11) in vitro, as well as rainbow trout challenged with Aeromonas salmonicida- or flagellin-stimulated Atlantic salmon in vivo. The presence of SAA protein was analysed in RTS-11 cell line supernatants, liver, and spleen samples using ELISA, immunoblotting, and immunohistochemistry. This study is the first to characterise SAA protein levels in salmonids in vivo and in vitro. The newly developed salmonid SAA antibody was able to discriminate between stimulated and unstimulated specimens, showing that it can be used to study the acute-phase response in salmonids with the potential to be further developed into assays to monitor and evaluate health in wild and farmed fish.
- Subjects
AMYLOID; IMMUNOGLOBULINS; RAINBOW trout; ATLANTIC salmon; RECOMBINANT proteins; DATA protection
- Publication
Cells (2073-4409), 2023, Vol 12, Issue 16, p2097
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells12162097