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- Title
Microvalve Bioprinting of MSC-Chondrocyte Co-Cultures.
- Authors
Dudman, Joseph; Ferreira, Ana Marina; Gentile, Piergiorgio; Wang, Xiao; Dalgarno, Kenneth
- Abstract
Recent improvements within the fields of high-throughput screening and 3D tissue culture have provided the possibility of developing in vitro micro-tissue models that can be used to study diseases and screen potential new therapies. This paper reports a proof-of-concept study on the use of microvalve-based bioprinting to create laminar MSC-chondrocyte co-cultures to investigate whether the use of MSCs in ACI procedures would stimulate enhanced ECM production by chondrocytes. Microvalve-based bioprinting uses small-scale solenoid valves (microvalves) to deposit cells suspended in media in a consistent and repeatable manner. In this case, MSCs and chondrocytes have been sequentially printed into an insert-based transwell system in order to create a laminar co-culture, with variations in the ratios of the cell types used to investigate the potential for MSCs to stimulate ECM production. Histological and indirect immunofluorescence staining revealed the formation of dense tissue structures within the chondrocyte and MSC-chondrocyte cell co-cultures, alongside the establishment of a proliferative region at the base of the tissue. No stimulatory or inhibitory effect in terms of ECM production was observed through the introduction of MSCs, although the potential for an immunomodulatory benefit remains. This study, therefore, provides a novel method to enable the scalable production of therapeutically relevant micro-tissue models that can be used for in vitro research to optimise ACI procedures.
- Subjects
BIOPRINTING; CO-cultures; MEDICAL screening; HIGH throughput screening (Drug development); TISSUE culture; CARTILAGE cells; PLANT tissue culture
- Publication
Cells (2073-4409), 2021, Vol 10, Issue 12, p3329
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells10123329