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- Title
Peritoneal inflammation and fibrosis in C-reactive protein transgenic mice undergoing peritoneal dialysis solution treatment.
- Authors
Poon, Peter Yam ‐ Kau; Lan, Hui ‐ Yao; Kwan, Bonnie Ching ‐ Ha; Huang, Xiao ‐ Ru; Chow, Kai ‐ Ming; Szeto, Cheuk ‐ Chun; Li, Philip Kam ‐ Tao
- Abstract
Aim C-reactive protein (CRP) is a mediator of systemic inflammation. Peritoneal dialysis (PD) is known to cause peritoneal inflammation and fibrosis. We compare the degree of peritoneal inflammation and fibrosis in wild-type (WT) and CRP-transgenic (Tg) mice after PD treatment. Methods WT ( n = 7) and CRP-Tg ( n = 10) C57BL/6 J mice (all male, 10-12 weeks old) were injected intra-peritoneally with 4.25% dextrose PD solution (3 mL/mouse) daily for 28 days, followed by a 2-h peritoneal equilibration test (PET). The mice were then killed. Parietal peritoneal and omental tissues were collected for the assessment of inflammation and fibrosis. Results After 28 days of PD treatment, CRP-Tg mice had higher dialysate-to-plasma (D/P) creatinine ratio than that of WT mice. Parietal peritoneum of the CRP-Tg mice was more cellular and thicker than that of the WT mice. CRP-Tg mice also had higher connective tissue growth factor (CTGF), intercellular adhesion molecule 1 (ICAM1) and tumor necrosis factor α (TNFα) RNA expressions as well as immunohistochemical staining in the parietal peritoneum than that of the WT mice. Conclusions CRP-Tg mice have significantly more inflammation and fibrosis than WT mice after PD treatment. Our results suggest that CRP play a role in inflammation and fibrosis induced by PD. The implication of our results to human PD therapy needs further investigations.
- Subjects
KIDNEY disease risk factors; PERITONEAL dialysis; FIBROSIS; C-reactive protein; CONNECTIVE tissue growth factor
- Publication
Nephrology, 2017, Vol 22, Issue 2, p125
- ISSN
1320-5358
- Publication type
Article
- DOI
10.1111/nep.12741