We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Induction of BAG2 protein during proteasome inhibitor-induced apoptosis in thyroid carcinoma cells.
- Authors
Wang, H.-Q.; Zhang, H.-Y.; Hao, F.-J.; Meng, X.; Guan, Y.; Du, Z.-X.
- Abstract
<bold>Background and Purpose: </bold>Proteasome inhibitors exhibit cytotoxic against tumours of different histology. However, the mechanism of apoptosis induction by these compounds remains unclear and is likely to be a complex cascade of events. Bcl-2-associated athanogene (BAG) family proteins are characterized by their property of interaction with a variety of partners involved in modulating the proliferation/death balance, including heat shock proteins (HSP), Bcl-2, Raf-1. The role of BAG family proteins in proteasome inhibition has not been elucidated.<bold>Experimental Approach: </bold>Effects of proteasome inhibitors on BAG2 expression were evaluated using real-time reverse transcription-polymerase chain reaction (RT-PCR). BAG2 expression was knocked down by small interfering RNAs (siRNA). Cell death was evaluated using Annexin V/propidium iodide staining and subsequent FACS.<bold>Key Results: </bold>The proteasome inhibitors, MG132, PSI, lactacystin and epoxomicin, induced BAG2 at the transcriptional level. MG132-induced apoptosis was significantly suppressed by BAG2 knockdown using RNA interference.<bold>Conclusions and Implications: </bold>Our results suggest that BAG2 is a novel molecule induced by proteasome inhibition, which exhibits a pro-apoptotic property in death of thyroid cancer cells induced by proteasome inhibition.
- Subjects
THYROID cancer; CANCER cells; HEAT shock proteins; APOPTOSIS; POLYMERASE chain reaction; ANTINEOPLASTIC agents; RNA; CELL death
- Publication
British Journal of Pharmacology, 2008, Vol 155, Issue 5, p655
- ISSN
0007-1188
- Publication type
journal article
- DOI
10.1038/bjp.2008.302