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- Title
Genetic polymorphism of casein alpha-S1 gene in Tunisian local Goat.
- Authors
Jemmali, Borni; Kamoun, Mounir; Haddar, Marwa; Gara, Abderrahmene Ben; Selmi, Houcine; Hammami, Moncef; Amraoui, Marouene; Rouissi, Hamadi; Boulbaba, Rekik
- Abstract
The genetic polymorphism of the casein alpha-S1 locus was investigated in Tunisian goat. Blood samples were collected from local goat breed. Genomic DNA samples were obtained from leukocytes of 75 dairy goats and regions of interest in the gene were amplified through Polymerase Chain Reaction (PCR), then evaluated in agarose gels. For better characterization of the single nucleotide polymorphism, if exist, a PCR-Restriction Fragment Length Polymorphism study was performed employing the endonuclease XmnI. DNA amplification using primers produced fragments with sizes of 457 bp. The PCR products of primer (223 bp) digested by restriction enzyme Xmn1 produced four fragments at 223 bp, 212bp, 161bp and 150-bp. The results showed that local goat breed had different genotypes A/A, B/C, C/C and DD. Our results revealed that the CSN1S1 allelic variants in tested breed showed different genotypes, three of them were homozygous 12.5%, 60.5% and 12.8% respectively for A/A, C/C and D/D and the other was heterozygous B/C (14.2%). Identification of different variants of the casein alpha-S1 can be used to improve milk quality of local goat breed.
- Subjects
TUNISIA; GOAT genetics; GENETIC polymorphisms; LEUCOCYTES; POLYMERASE chain reaction; RESTRICTION fragment length polymorphisms; GENOTYPES
- Publication
Biomirror, 2012, Vol 3, Issue 11, p48
- ISSN
0976-9080
- Publication type
Article