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- Title
Optimization of phenolic compound recovery and antioxidant activity from carob pulp using response surface methodology.
- Authors
Saci, F.; Louaileche, H.; Bachir bey, M.; Meziant, L.
- Abstract
The optimum extraction conditions for recovery of total phenolics and antioxidant activity of carob pulp were analysed using response surface methodology. The effects of acetone concentration (60-80%), extraction time (60-120 min), extraction temperature (50-90°C) and sample/solvent ratio (10-40 mg/10mL) were investigated. Second order polynomial models produced a satisfactory fitting of the experimental data with regard to total phenolic content (R² = 0.9949, P<0.0001) and antioxidant activity (R² = 0.9947, P<0.0001). Response surface analysis showed that the optimal extraction parameters that maximized antioxidant extraction were acetone concentration of 70.85%, extraction time of 101.50 min, temperature of 88.35°C and sample/solvent ratio of 16.65mg/10mL. The optimum values for total phenolic and antioxidant activity were 2557.96 mg GAE/100g and 2393.75mgGAE/100g, respectively. The experimental values agreed well with those predicted, indicating the suitability of the model and the success of response surface methodology in optimizing the extraction conditions.
- Subjects
PHENOLS; ANTIOXIDANTS; CAROB; ACETONE; RESPONSE surfaces (Statistics)
- Publication
International Food Research Journal, 2017, Vol 24, Issue 3, p1094
- ISSN
1985-4668
- Publication type
Article