We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Spleen extracellular matrix provides a supportive microenvironment for β-cell function.
- Authors
Khorsandi, Layasadat; Orazizadeh, Mahmoud; Nejad, Darioush Bijan; Moghadam, Abbas Heidari; Nejaddehbashi, Fereshteh; Fard, Yousef Asadi
- Abstract
Objective(s): Type 1 diabetes mellitus is a common autoimmune and multifactorial disorder. Researchers have been interested in making a favorable islet-like tissue model for the treatment of diabetes. The main objective of this study was to determine the effects of the spleen extracellular matrix (S-ECM) on the function of the MIN6 cell line (a β-cell model). Materials and Methods: In this experimental research, Wistar rat spleens were decellularized by sodium dodecyl sulfate (SDS) and Triton X-100. S-ECM was characterized by histological assessments, scanning electron microscopy, determination of residua DNA, and examination of the mechanical tensile property. Then, MIN6 cells were seeded on S-ECM scaffold. Glucose-stimulated insulin secretion and mRNA expression of insulin-related genes were examined to confirm the function of the cells. Results: The main components of S-ECM such as collagen and glycosaminoglycan remained after decellularization. Furthermore, very low residual DNA and appropriate mechanical behavior of S-ECM provided an ideal extracellular microenvironment for the MIN6 cells. GSIS results showed that the seeded cells in S-ECM secreted more insulin than the traditional two-dimensional (2D) culture. The expression of specific insulin-related genes such as PDX-1, insulin, Maf-A, and Glut-2 in the recellularized scaffold was more significant than in the 2D traditional cultured cells. Also, MTT assay results showed that S-ECM were no cytotoxic effects on the MIN6 cells. Conclusion: These results collectively have evidenced that S-ECM is a suitable scaffold for stabilizing artificial pancreatic islands.
- Subjects
EXTRACELLULAR matrix; TYPE 1 diabetes; SPLEEN; SODIUM dodecyl sulfate; ARTIFICIAL islands
- Publication
Iranian Journal of Basic Medical Sciences, 2022, Vol 25, Issue 9, p1159
- ISSN
2008-3866
- Publication type
Article
- DOI
10.22038/IJBMS.2022.65233.14360