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- Title
Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China.
- Authors
Yili Chen; Penghao Guo; Han Huang; Yongxin Huang; Zhongwen Wu; Kang Liao
- Abstract
Objective(s): Acinetobacter pittii has become an emerging opportunistic noscomial pathogen worldwide with multi-drug resistance. In the present study, an A. pittii strain was isolated from bronchoalveolar lavage fluid sample harboring both OXA-58 and NDM-1carbapenemase producing genes. The mechanisms of carbapenem resistance of the A. pittii strain was investigated. Materials and Methods: Carbapenemase producing genes were examined by PCR and DNA sequencing. S1-PFGE was used to localize carbapenemase encoding genes. Filter mating and electrotransformation were used to investigate the transferability of such carbapenemase encoding genes between different strains. Genetic surroundings of blaOXA-58 and blaNDM-1 genes were detected as well. Results: The A. pittii strain, carrying both OXA-58 and NDM-1 carbapenemase encoding genes, was resistant to all β-lactam antibiotics, while suscepitible to ciprofloxacin, levofloxacin, tobramycin, cotrimoxazole and tigecycline. Southern blot hybridization for the blaOXA-58 and blaNDM-1 gene indicated that the two genes locate in the same plasmid with molecular weight of 310.1-336.5kb. BlaOXA-58 was located in an ISAba3-blaOXA-58-ISAba3-like structure, and the blaNDM-1 gene cluster was embedded in an ISAba125-aphA6-blaNDM-1-bleMBL-ΔtrpF-dsbC-cutA structure sequentially. Conclusion: In the present study, it is first reported an A. pittii clincal strain in China, co-harboring OXA-58 and NDM-1 carbapenemase producing genes residing on a same plasmid. In hospital and community settings, it is of great significance and urgence to increase the surveillance of these kinds of organisms.
- Subjects
CHINA; BRONCHOALVEOLAR lavage; GENE clusters; BETA lactamases; VIRULENCE of Escherichia coli; MULTIDRUG resistance; CARBAPENEMASE
- Publication
Iranian Journal of Basic Medical Sciences, 2019, Vol 22, Issue 1, p106
- ISSN
2008-3866
- Publication type
Article
- DOI
10.22038/ijbms.2018.28934.6994