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- Title
AN EVALUATION OF DIRECT MICROSCOPY EXAMINATION AND CULTURE SENSITIVITY IN DIAGNOSIS OF DERMATOPHYTOSIS.
- Authors
Hashemi, Seyed Jamal; Daie-Ghazvini, Roshanak; Zibafar, Ensieh; Geramishoar, Mohsen; Borujeni, Zeinab Borjian; Hosseinpour, Leila; Zareei, Mahdi; Mirhendi, Hossein
- Abstract
Introduction and Objectives: Diseases of skin and its appendixes (hair and nail) are caused by two groups of infectious and non-infectious agents. The former agents consist of fungi, bacteria, viruses and others (e. g insects). The most important fungi in these diseases are dermatophytes, Malassezia, Candida and non-dermatophytes. Each one of infectious agents have specific characteristics and particular treatment, also, in the fungal diseases each causative genus (even, sometimes each causative species) have different sensitivity to antifungal therapy; therefore, identifying causative agent is necessary. Thus, applying a sensitive and confidential laboratory method for rapid and non-expensive diagnosis can be useful and it is necessary to prefer and give priority to specific method based on laboratory documents. The common and available laboratory diagnosis methods are DME and culture, thus, the aim of this study is to determine the priority of these two methods to diagnosis (not to identify) dermatophytosis Materials and Methods: This study has been accomplished by checking the information of referred persons to the laboratory of department of medical mycology, school of public health and institute of public health research, Tehran University of Medical sciences in Iran, to diagnose of clinical suspected dermatophytosis. Results: From the beginning to the end of 2011, information survey of 1601 persons were revealed that 301 persons of them were infected by dermatophytes, the DME and culture results of these patients have been divided to several groups and stated in below. G1: DME positive and culture positive = 186 (61/8%) cases, G2: DME positive and culture negative = 110 (36/5%) cases, G3: DME negative and culture positive = 5 (1/7%) cases, G4: DME positive or culture positive (total of cases) = 301 (100%) cases. Conclusion: Although culture is necessary for identification of dermatophytes, but, the important matter is only diagnosis of disease, the DME- at least in our laboratory- has high sensitivity (36/5% more than culture). In conclusion, to achieve two aims (diagnosis and identifying), both methods (DME and culture) are necessary.
- Subjects
RINGWORM; SKIN disease diagnosis; FUNGI; BACTERIA; VIRUSES; ANTIFUNGAL agents; DIAGNOSIS
- Publication
Jundishapur Journal of Microbiology, 2013, p69
- ISSN
2008-3645
- Publication type
Article