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- Title
GENETIC DIVERSITY AMONG IRANIAN CANDIDA ALBICANS ISOLATED FROM NAILS AND SKIN.
- Authors
Katiraee, Farzad; Shadi, Sahar; Farshi, Yashar; Fakhri, Omid; Zare, Payman
- Abstract
Introduction and Objectives: Candida albicans is the most prevalent yeast species that causes fungal infection. Also, C. albicans is one of the most frequent etiologic agents of dermatomycosis and onychomycosis. In spite of the new antifungals and health care, the incidence of mycoses has increased markedly, especially in immunocompromised individuals. The aim of this study is assessing the genotypic diversity and genetic relationship of C. albicans isolated from nail and skin. Materials and Methods: The genetic diversity of 50 clinical and reference strains of C. albicans from nails and skin clinical disorders were studied through randomly amplified polymorphic DNA (RAPD) techniques. C. albicans isolates were diagnosed by conventional and PCR- RFLP method. RAPD-PCR was optimized using three primers of arbitrary sequences. Cluster analysis of PCR product on agarose gel was performed using UPGMA method. Results: C. albicans isolates generated up to15 bands and were grouped in 27 genotypes depending on the primers used. RAPD profiles identified 7-9 clusters of genotype, based on primer used, among the 50 isolates. Conclusion: Our results revealed moderate genetic diversity among nail and skin isolates, and no association with their origin and the clinical characteristics. RAPD was suitable for fingerprinting analysis and was found to generate the reproducible fingerprints, yielding well-resolved banding patterns. RAPD fingerprinting can be used to genotype C. albicans. For obtaining reliable results by RAPD method, using more than one primer and comparative analysis of these primers are appropriate.
- Subjects
CANDIDA albicans; DERMATOMYCOSES; ONYCHOMYCOSIS; ANTIFUNGAL agents; MYCOSES; DISEASE incidence; CLUSTER analysis (Statistics); RAPD technique
- Publication
Jundishapur Journal of Microbiology, 2013, p61
- ISSN
2008-3645
- Publication type
Article