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- Title
Rainbow trout cell bioassay-derived relative potencies for halogenated aromatic hydrocarbons: comparison and sensitivity analysis
- Authors
Giesy, J. P.; Richter, C. A.; Villeneuve, D. L.; Blankenship, A. L.
- Abstract
Rainbow trout hepatoma cells, stably transfected with a luciferase reporter gene under control of dioxin-responsive elements (RLT 2.0 cells) were used to derive relative potencies (RPs) for a variety of halogenated aromatic hydrocarbons (HAHs) that are structurally similar to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). This in vitro bioassay utilizes 96-well microplates, which provide high sample throughput and assay efficiency without affecting sensitivity. The RLT 2.0-derivedpotencies for dioxin and furan congeners, relative to 2,3,7,8-TCDD, ranged from 0.917 for 1,2,3,4,7,8-hexachlorodibenzofuran to 0.208 for1,23.7.8pentachlorodibenzofuran. All mono- and di-ortho polychlorinated biphenyls (PCBs) tested had RPs that were orders of magnitude less than TCDD, but point estimates could not be determined. The RLT 2.0-derived RPs were found to be comparable to both other rainbow trout-specific RPs and RPs based on mammalian bioassays. Sensitivity analysis suggested that the range of uncertainty associated with TCDD equivalent (TEQ) estimates based on RLT 2.0-derived RPs is approximately 10-fold. Within this degree of uncertainty and the context of this study, the RLT 2.0 bioassay showed no definitive biases or inaccuracies relative to similar mammalian- or 4h-specific in vitro bioassays. Thus, the RLT 2.0 bioassay appears to be a useful tool for evaluating dioxinlike potency of HAHs to fish.
- Subjects
POLLUTION; POLYCHLORINATED dibenzofurans; POLYCHLORINATED dibenzodioxins; FISHES; POLYCHLORINATED biphenyls
- Publication
Environmental Toxicology & Chemistry, 1999, Vol 18, Issue 5, p879
- ISSN
0730-7268
- Publication type
Article