We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
IncFIB plasmids carrying the resistance gene bla<sub>CTX-M-15</sub> in ESBL-producing Escherichia coli clones from pediatric patients.
- Authors
Rocha-Gracia, Rosa del Carmen; Lozano-Zarain, Patricia; Gutiérrez Cázarez, Zita; Andrea Alonso, Carla; Brambila, Eduardo; Torres, Carmen; Cortés-Cortés, Gerardo
- Abstract
Introduction: The emergence of extended-spectrum ß-lactamases (ESBLs)-producing Escherichia coli clones are a public health concern worldwide. Scarce information does exist about the spread of ESBLs-producing E. coli in pediatric patients from developing countries. Methodology: E. coli strains were analyzed by multilocus-sequence-typing, pulsed-field-gel-electrophoresis and phylogenetic group. The antimicrobial-resistance genes were detected by PCR, and plasmid content by the PCR-based replicon-typing. Horizontal transfer was tested by conjugation and the location of the blaCTX-M-15 gene by Southern blot hybridization. Results: Thirty-two cefotaxime-resistant E. coli were recovered. Eleven of them were ESBL-producing isolates, which were well characterized and ascribed to seven sequence types and five phylogroups. The ESBL CTX-M-15 was the most prevalent enzyme (9 of 11). Plasmids of variable sizes (40-220 kb) were visualized, and the incompatibility (Inc) group FIB plasmid-replicon was detected in the ESBL strains and transferred by conjugation in 45.45% of them. Plasmid-borne toxin-antitoxin systems were the most frequently detected systems, strongly associated to IncF plasmids. The CTX-M-15-encoding gene was located on IncFIB plasmids. Conclusions: Even though a small number of ESBL-producing strains was recovered, we evidenced that IncFIB plasmids carry the blaCTX-M-15 gene, highlighting the role of IncF-type plasmids in facilitating the spread and maintenance of ESBL-encoding genes, which further favors the rapid increase of the antimicrobial resistance dissemination in disease-causing E. coli strains in pediatric patients.
- Subjects
BETA lactamases; KLEBSIELLA pneumoniae; CHILD patients; MOLECULAR cloning; PLASMIDS; ESCHERICHIA coli; GENES
- Publication
Journal of Infection in Developing Countries, 2022, Vol 16, Issue 3, p500
- ISSN
2036-6590
- Publication type
Article
- DOI
10.3855/jidc.15080