We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Targeted Delivery of Salusin‐α Into Rabbit Carotid Arterial Endothelium Using SonoVue.
- Authors
Wang, Yuxue; Luo, Min; Mao, Xiaolu; Shi, Xiaoyan; Liu, Xiang
- Abstract
Objectives: A new method based on the adhesion of SonoVue to plasmids was assessed to achieve targeted gene delivery into the vascular endothelium. Methods: pEGFP‐Salusin‐α and pcDNA3.1‐Salusin‐α plasmids were transfected into the arterial endothelium of different rabbit groups. Western blotting was performed to analyze the expression of EGFP and salusin‐α in the common carotid arteries of rabbits from different groups, and ELISA was performed to detect plasma salusin‐α levels in rabbits from each group; simultaneously, blood parameters of different groups of rabbits were measured. Results: Green fluorescence was observed in the right common carotid artery of rabbits transfected with pEGFP‐Salusin‐α, but not in the endothelial cells of not‐transfected control rabbits. The expression of salusin‐α in the transfected animals was higher than that in the control not‐transfected animals (P <.05). In rabbits transfected with pcDNA3.1‐Salusin‐α plasmid, salusin‐α expression was higher than in the not‐transfected control animals (P <.05). However, there was no significant difference in plasma salusin‐α levels between transfected animals and controls (P >.05). Blood parameters were also measured in both groups. Conclusions: Our data confirm the establishment of a new method using SonoVue for targeted gene delivery into the arterial endothelium. Our study outcomes propose a new method of intervention in atherosclerosis and a new tool for targeted gene delivery.
- Subjects
ENDOTHELIUM; VASCULAR endothelium; RABBITS; ENDOTHELIAL cells; WESTERN immunoblotting; CAROTID artery; INTERNAL carotid artery
- Publication
Journal of Ultrasound in Medicine, 2022, Vol 41, Issue 2, p365
- ISSN
0278-4297
- Publication type
Article
- DOI
10.1002/jum.15714