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- Title
Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
- Authors
Bigarella, Carolina L.; Vieira Ferro, Karla P.; Barcellos, Karin S.A.; Martins-de-Souza, Daniel; Traina, Fabiola; Novello, José C.; Olalla Saad, Sara T.; Archangelo, Leticia Fröhlich
- Abstract
Abstract: ARHGAP21 is a 217kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function.
- Subjects
RHO GTPases; CELL migration; POST-translational modification; CELL lines; IMMUNOPRECIPITATION; IMMUNOFLUORESCENCE; CELL proliferation
- Publication
FEBS Letters, 2012, Vol 586, Issue 19, p3522
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.08.012