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- Title
Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres.
- Authors
Chen, Wentao; Šmajs, David; Hu, Yongfei; Ke, Wujian; Pospíšilová, Petra; Hawley, Kelly L; Caimano, Melissa J; Radolf, Justin D; Sena, Arlene; Tucker, Joseph D; Yang, Bin; Juliano, Jonathan J; Zheng, Heping; Parr, Jonathan B
- Abstract
<bold>Background: </bold>Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples.<bold>Methods: </bold>We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China.<bold>Results: </bold>By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions.<bold>Conclusions: </bold>This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
- Subjects
CHINA; GUANGZHOU (China); TREPONEMA pallidum; SYPHILIS; SPIROCHETES; SUBSPECIES; DIAGNOSIS of syphilis; BACTERIA classification; RESEARCH; ANIMAL experimentation; RABBITS; COMPARATIVE studies; RESEARCH funding; BACTERIA
- Publication
Journal of Infectious Diseases, 2021, Vol 223, Issue 5, p848
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiaa449