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- Title
Nutritional and hormonal regulation of articular collagen production in diabetic animals.
- Authors
Umpierrez, Guillermo E.; Goldstein, Steven; Phillips, Lawrence S.; Spanheimer, Robert G.; Umpierrez, G E; Goldstein, S; Phillips, L S; Spanheimer, R G
- Abstract
Although changes in collagen production probably play a major role in the connective tissue defects of diabetes, we do not know to what extent these changes are attributable to hormonal/metabolic versus nutritional alterations. To study collagen production as influenced separately by nutrition versus hormonal/metabolic factors, rats were given 50 mg/kg i.v. streptozocin (STZ) (mild weight-gaining diabetes) or 100 mg/kg STZ (severe weight-losing diabetes) and compared with nondiabetic food-restricted rats to match weight changes in diabetic animals. Articular cartilage was incubated with [3H]proline, and uptake of [3H]proline into both collagen and noncollagen proteins was determined with purified bacterial collagenase. Collagen decreased to 49% in mildly diabetic rats and 16% in severely diabetic rats, compared with control rats fed ad libitum and decreased to 85 and 73%, respectively, in food-restricted rats (both P less than .01 vs. diabetes). Diabetes induced a greater defect in collagen production than food restriction and a greater decrease in collagen than noncollagen protein production within each group, suggesting a specific effect on collagen. With comparable levels of metabolic severity (glucose, beta-hydroxybutyrate), diabetic animals that lost weight produced significantly less collagen than animals that gained weight, suggesting separate mechanisms. Quantitation of the impact of undernutrition on collagen production in diabetes demonstrated that approximately 31 to 32% of the defect was due to undernutrition, leaving approximately 68-69% of the defect due to the diabetic state.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects
PROLINE metabolism; ANIMAL experimentation; ARTICULAR cartilage; COLLAGEN; COMPARATIVE studies; DIABETES; DYNAMICS; RESEARCH methodology; MEDICAL cooperation; NUTRITIONAL requirements; RATS; REFERENCE values; RESEARCH; RESEARCH funding; EVALUATION research
- Publication
Diabetes, 1989, Vol 38, Issue 6, p758
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.38.6.758