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- Title
Heterologous Biosynthesis of Health-Promoting Baicalein in Lycopersicon esculentum.
- Authors
Liao, Jingjing; Xie, Lei; Liu, Tingyao; Mo, Changming; Cui, Shengrong; Jia, Xunli; Huang, Xiyang; Luo, Zuliang; Ma, Xiaojun
- Abstract
Baicalein is a valuable flavonoid isolated from the medicinal plant Scutellaria baicalensis Georgi, which exhibits intensive biological activities, such as anticancer and antiviral activities. However, its production is limited in the root with low yield. In this study, In-Fusion and 2A peptide linker were developed to assemble SbCLL-7, SbCHI, SbCHS-2, SbFNSII-2 and SbCYP82D1.1 genes driven by the AtPD7, CaMV 35S and AtUBQ10 promoters with HSP, E9 and NOS terminators, and were used to engineer baicalein biosynthesis in transgenic tomato plants. The genetically modified tomato plants with this construct synthesized baicalein, ranging from 150 ng/g to 558 ng/g FW (fresh weight). Baicalein-fortified tomatoes have the potential to be health-promoting fresh vegetables and provide an alternative source of baicalein production, with great prospects for market application.
- Subjects
SCUTELLARIA; TOMATOES; TRANSGENIC plants; BIOSYNTHESIS; CHINESE skullcap; MEDICINAL plants
- Publication
Molecules, 2022, Vol 27, Issue 10, p3086
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27103086