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- Title
Identification and Study of Biomarkers from Novichok-Inhibited Butyrylcholinesterase in Human Plasma.
- Authors
Jeong, Woo-Hyeon; Lee, Jin-Young; Lim, Kyoung-Chan; Kim, Hyun-Suk
- Abstract
To identify biomarkers of ethyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A234)- or methyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A232)-inhibited butyrylcholinesterase (BChE), we investigated nonapeptide adducts containing the active site serine, which plays a key role in enzyme activity, using LC-MS/HRMS. Biomarkers were acquired as expected, and they exhibited a significant amount of fragment ions from the inhibiting agent itself, in contrast to the MS2 spectra of conventional nerve agents. These biomarkers had a higher abundance of [M+2H]2+ ions than [M+H]+ ions, making doubly charged ions more suitable for trace analysis.
- Subjects
BUTYRYLCHOLINESTERASE; NERVE gases; BIOMARKERS; DAUGHTER ions; CHEMICAL warfare agents
- Publication
Molecules, 2021, Vol 26, Issue 13, p3810
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules26133810